A new TaqI polymorphism of the complement factor B gene
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چکیده
منابع مشابه
A new TaqI polymorphism of the complement factor B gene.
A new TaqI polymorphism of the complement factor B gene SOURCE/DESCRIPTION : A full length factor B cDNA insert from clone pFB3B (1) which detects the TaqI polymorphism previously described at the C2 and BF loci (2). POLYMORPHISM : TaqI (TjCGA) identifies a new allele resulting from an additional site subdividing the 3.1 kb fragment of the BF gene (2) into two fragments of 1.9 and 1.2 kb. CHROM...
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Protocol: Reactions were performed as described (2) using 1 ng of DNA and 100 pmoles of each primer. DNA was amplified for 20 cycles using a Perkin Elmer Cetus DNA Thermal Cycler: denaturation, 94°C, 1 min; annealing, 60°C, 1 min; and extension, 70°C, 1.5 min. Ten /il of the reaction was analyzed directly on a 2.5 % NuSieve GTG agarose gel run in Tns-borate buffer. The amplified DNA fragments v...
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References: l)Nussbaum,R.L., Airhart,S.D. and Ledbetter.D.H. (1986) Am. J. Med. Genet. 23, 457-466. 2)Patterson,M.N., Bell,M.V., BloomfieldJ,, Flint,T., Dorkins.H., Thibodeau.S.N., Schaid,D., Bren,G., Schwartz,C.E., Wieringa,B., Ropers,H.-H., Callen.D.F., Sutherland.G., Froster-Iskenius.U., Vissing.H. and Davies,K.E. (1989) Genomics 4, 570-578. A Taql polymorphism adjacent to the factor VIII ge...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1988
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/16.24.11851